Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.B10 (
caspase-7
)
896
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Werner syndrome
(WS) is a rare autosomal progeroid disorder caused by a mutation in the gene encoding the
WRN
(Werner syndrome protein), a member of the RecQ family of helicases with a role in maintaining genomic stability. Genetic association studies have previously suggested a link between
WRN
and susceptibility to benzene-induced hematotoxicity. To further explore the role of
WRN
in benzene-induced hematotoxicity, we used short hairpin RNA to silence endogenous levels of
WRN
in the human HL60 acute promyelocytic cell line and subsequently exposed the cells to hydroquinone (HQ). Suppression of
WRN
led to an accelerated cell growth rate, increased susceptibility to hydroquinone-induced cytotoxicity and genotoxicity as measured by the single-cell gel electrophoresis assay, and an enhanced DNA damage response. More specifically, loss of
WRN
resulted in higher levels of early apoptosis, marked by increases in relative levels of cleaved
caspase-7
and cleaved poly (ADP-ribose) polymerase 1, in cells treated with HQ compared with control cells. Our data suggests that
WRN
plays an important role in the surveillance of and protection against DNA damage induced by HQ. This provides mechanistic support for the link between
WRN
and benzene-induced hematotoxicity.
...
PMID:Werner syndrome protein, WRN, protects cells from DNA damage induced by the benzene metabolite hydroquinone. 1906 79
