Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.65 (
Der p 1
)
346
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The major
allergen Der p 1
of the house dust mite Dermatophagoides pteronyssinus is a papain-like cysteine protease (
CA1
) produced as an inactive precursor and associated with allergic diseases. The propeptide of
Der p 1
exhibits a specific fold that makes it unique in the
CA1
propeptide family. In this study, we investigated the activation steps involved in the maturation of the recombinant protease
Der p 1
expressed in Pichia pastoris and the interaction of the full-length and truncated soluble propeptides with their parent enzyme in terms of activity inhibition and BIAcore interaction analysis. According to our results, the activation of protease
Der p 1
is a multistep mechanism that is characterized by at least two intermediates. The propeptide strongly inhibits unglycosylated and glycosylated recombinant
Der p 1
(K(D)=7 nM) at neutral pH. This inhibition is pH dependent. It decreases from pH 7 to pH 4 and can be related to conformational changes of the propeptide characterized by an increase of its flexibility and formation of a molten globule state. Our results indicate that activation of the zymogen at pH 4 is a compromise between activity preservation and propeptide unfolding.
...
PMID:Relationship between propeptide pH unfolding and inhibitory ability during ProDer p 1 activation mechanism. 1791 63
