Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.65 (
Der p 1
)
346
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
It has been well documented that the mite Dermatophoides pteronyssinus (Der p) is the major allergen in house dust in Taiwan. The purpose of this study is to develop a sandwich immunometric assay which can be used for the standardization of mite allergen extracts or house dust, for assessing the procedures of mite avoidance, and for identifying species in epidemiological studies.
Der p 1
allergen (both recombinant and native
Der p 1
) was purified and a panel of
Der p 1
specific monoclonal antibodies was developed. The monoclonal antibodies were biotinylated and a two-site enzyme-linked immunosorbent assay (ELISA) with
peroxidase
-conjugated streptavidin was developed for the detection of
Der p 1
allergen. The results suggest that these monoclonal antibodies could be applied both in affinity columns for the purification of native
Der p 1
antigen and in sandwich-ELISA for epidemiological study.
...
PMID:Preparation of Der p 1 specific monoclonal antibodies and use in a two-site-ELISA to detect Der p 1 allergen. 1091 77
