Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.65 (
Der p 1
)
346
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Although mite major group 1 allergens,
Der p 1
and
Der f 1
, were first isolated as cysteine proteases, some studies reported that natural
Der p 1
exhibits mixed cysteine and
serine protease
activity. Clarifying whether the
serine protease
activity originates from
Der p 1
or is due to contamination is important for distinguishing between the pathogenic proteolytic activities of group 1 allergens and mite-derived serine proteases. Recombinant mite group 1 allergens would be useful tool for addressing this issue, because they are completely free from contamination by mite serine proteases. Recombinant
Der p 1
and
Der f 1
, and highly purified natural forms exhibited only cysteine protease activity. However, commercially available natural forms exhibited both activities, but the two activities were eluted into different fractions in size-exclusion column chromatography. The substrate specificity associated with the
serine protease
activity was similar to that of Der f 3. These results indicate that the
serine protease
activity does not originate from group 1 allergens.
...
PMID:Recombinant Der p 1 and Der f 1 exhibit cysteine protease activity but no serine protease activity. 1570 69
Several cysteine and
serine protease
allergens have been cloned from house dust mites, including
Der p 1
, Der p 3, Der p 6, and Der p 9. A significant body of evidence suggests that these allergens mimic helper T (Th) 2 cell adjuvants.
Der p 1
cleaves CD23 from activated B cells and CD25 from T cells.
Der p 1
proteolytically degrades tight junctions in lung epithelium and causes release of proinflammatory cytokines from bronchial epithelial cells, mast cells, and basophils. These synergistic effects of mite enzyme allergens may promote IgE synthesis and have direct inflammatory effects on lung epithelium, which could explain why mite allergens are associated with asthma. The crystal structures of the proenzyme and mature forms of
Der p 1
have been determined, as have the structures of other indoor allergens that are not enzymes (eg, Der p 2, Fel d 1, and Bla g 2). Cockroach allergens are strongly associated with asthma in US inner cities, yet none of the cockroach allergens that have been cloned are proteolytic enzymes. Thus although mite proteases allergens may act as Th2 adjuvants, a paradoxical effect is that other allergens may elicit strong Th2 responses in the absence of enzyme activity.
...
PMID:Proteases as Th2 adjuvants. 1769 45
