Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.65 (
Der p 1
)
346
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The potent allergenicity of
Der p 1
, a major allergen of the house dust mite Dermatophagoides pteronyssinus, is thought to be related to its cysteine protease activity. Therefore, there is considerable interest in developing a sensitive assay for measuring
Der p 1
activity to screen for specific inhibitors. This study demonstrates for the first time that the activity of
Der p 1
can be measured conveniently in a continuous rate assay with the fluorogenic substrate Boc-Gln-Ala-Arg-
AMC
(K(m) = 280 microM and kcat/K(m) = 4.6 x 10(3)/M/s).
...
PMID:A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermatophagoides pteronyssinus. 989 50
