Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.65 (
Der p 1
)
346
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
cDNA clones encoding a major house dust mite allergen,
Der f 1
, were isolated from a Dermatophagoides farinae cDNA library by
plaque
immunoscreening using rabbit anti-
Der f 1
serum. The sequences cover the complete open reading frame encoding the prepro-form. The sequence is different from previously reported cDNA of
Der f 1
in six bases and the encoded amino acid sequence is different in two residues. Pro-forms of
Der f 1
and its mutant, in which the N-glycosylation motif was disrupted, expressed in Pichia pastoris were converted to the mature forms by an in vitro activation process and they showed significant IgE-binding. The biologically active rDer f 1 molecules would be useful for diagnostic testing and allergen-specific immunotherapy. In contrast,
Der f 1
directly expressed in Escherichia coli without the prosequence had very low IgE binding. The hypoallergenic
Der f 1
polypeptide could be useful for safer and more effective immunotherapy.
...
PMID:Cloning and expression of cDNA encoding the complete prepro-form of an isoform of Der f 1, the major group 1 allergen from house dust mite Dermatophagoides farinae. 1133 Jun 69
