Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.62 (
caspase-9
)
7,507
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The role and clinical implication of the
WWP2
E3 ubiquitin ligase in liver cancer are poorly understood. In the current study, we investigated the expression level of
WWP2
and its functions in cell adhesion, invasion, and migration in liver cancer. We used real-time PCR to detect the expression of
WWP2
in liver cancer and adjacent samples from the People's Hospital of Lishui and also analyzed The Cancer Genome Atlas (TCGA) RNA-seq data by bioinformatics. Migration and invasion were detected by transwell analysis. We detected a strong
WWP2
expression in tumor tissues of the People's Hospital of Lishui, and the survival rate was significantly higher in patients with lower
WWP2
-expressing tumors.
WWP2
small hairpin RNA (shRNA) lentivirus stably infected cells (shWWP2), Huh7, showed slower growth speed compared with scramble control-infected cells in a xenograft mouse model. Knockdown of
WWP2
Huh7 and BEL-7404 cells demonstrated a reduction in adhesion, invasion, and migration. Gene set enrichment analysis (GSEA) showed that
WWP2
is positively correlated to cancer-related pathways including the chemokine signaling pathway.
WWP2
also regulated MMP-9,
caspase-9
, CXCR3, and CCR5 expression in liver cancer cells. In addition, knockdown of CXCR3 and CCR5 significantly inhibited cell proliferation, adhesion, invasion, and migration in Huh7 and BEL-7404 cells. Our data suggest that targeting of
WWP2
may be a therapeutic strategy for liver cancer treatment.
...
PMID:Silencing of WWP2 inhibits adhesion, invasion, and migration in liver cancer cells. 2666 6
