Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.62 (
caspase-9
)
7,507
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Nicotinamide N-methyltransferase
(
NNMT
) has been found involved in cell proliferation of several malignancies. However, the functional role of
NNMT
in breast cancer has not been elucidated. In the present study, we showed that
NNMT
was selectively expressed in some breast cancer cell lines, down-regulation of
NNMT
expression in Bcap-37 and MDA-MB-231 cell lines by
NNMT
shRNA significantly inhibited cell growth in vitro, decreased tumorigenicity in mice and induced apoptosis. The silencing reciprocal effect of
NNMT
was confirmed by over-expressing
NNMT
in the MCF-7 and SK-BR-3 breast cancer cell lines which lack constitutive expression of
NNMT
. In addition, down-regulation of
NNMT
expression resulted in reducing expression of Bcl-2 and Bcl-xL, up-regulation of Bax, Puma, cleaved
caspase-9
, cleaved caspase-3 and cleaved PARP, increasing reactive oxygen species production and release of cytochrome c from mitochondria, and decreasing the phosphorylation of Akt and ERK1/2. These data suggest that down-regulation of
NNMT
induces apoptosis via the mitochondria-mediated pathway in breast cancer cells.
...
PMID:Down-regulation of nicotinamide N-methyltransferase induces apoptosis in human breast cancer cells via the mitochondria-mediated pathway. 2455 88
