Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.62 (
caspase-9
)
7,507
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Citrin is a mitochondrial aspartate-glutamate carrier primarily expressed in liver. Adult-onset type II citrullinemia is caused by mutations in the SLC25A13 gene that encodes for
citrin
, and patients with this condition do not express
citrin
. We found apoptotic hepatocytes in one such patient. This finding prompted us to investigate the role of
citrin
in hepatocyte survival. Knockdown of
citrin
by a vector-based short-hairpin RNA technique reduced cell viability and induced apoptosis of a hepatocellular carcinoma cell line, Hep3B cells. Caspase-3/7 and
caspase-9
were activated, and PARP was cleaved. Citrin knockdown also increased the expression of Bax and Bak, and reduced expression of Bcl-xL and Bcl-2. These alterations resulted in the release of cytochrome c from the mitochondria. Our results indicated that
citrin
downregulation induces apoptosis of hepatocytes through the mitochondrial death pathway, highlighting the importance of
citrin
in survival of hepatocytes and maintenance of liver function.
...
PMID:Downregulation of citrin, a mitochondrial AGC, is associated with apoptosis of hepatocytes. 2184 6
