Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.22.61 (caspase-8)
 6,833 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Objective To investigate the expression of apoptosis-related factors in diabetic nephropathy model rats after the intervention of dipeptidyl peptidase 4 (DPP-4) inhibitor sitagliptin. Methods ZDF(fa/fa) and ZDF(fa/+) rats were randomly divided into diabetes mellitus group and sitagliptin treatment group, and ZDF control mice (fa/+) were used as the control group. Non-fasting blood glucose levels were measured with glucose oxidase kit. Urine albumin level was detected by ELISA, and blood biochemical indexes were detected by automatic biochemical analyzer. The mRNA levels of tumor necrosis factor receptor 1(TNFR1), Fas, and caspase-3 in the renal tissue were examined by real-time fluorescence quantitative PCR, and the protein levels of DPP-4, GLP-1, TNFR1, caspase-3, and caspase-8 were detected by Western blot analysis. Results The levels of blood glucose, urinary microalbumin, blood urea nitrogen, serum creatinine increased in the diabetic group, and the levels of related indicators significantly decreased after treatment with sitagliptin. The mRNA levels of TNFR1 and caspase-3 in the diabetic rats went up and then down after the treatment with sitagliptin. The levels of DPP-4, TNFR1, caspase-3 and caspase-8 in the diabetic rats increased, while the level of GLP-1 decreased; after the treatment with sitagliptin, the corresponding protein content showed opposite changes. Conclusion Sitagliptin can inhibit inflammation and apoptosis in rats with diabetic nephropathy.
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PMID:[Sitagliptin inhibits cell apoptosis and inflammation of renal tissues in diabetic nephropathy model rats]. 3103 Jul 14