Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.61 (
caspase-8
)
6,833
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Idiopathic thrombocytopenic purpura (ITP)
is an organ specific autoimmune disorder in which T-lymphocyte abnormalities have pathogenetic importance. In a DNA microarray screen of CD3+ T-lymphocytes from
ITP
patients and healthy controls we found an altered expression of genes associated with apoptosis, e.g. A20,
caspase-8
and Bax. This together with our previous findings of increased gene expression of Fas, interferon-g and IL-2 receptor beta (IL2RB) indicated an altered activation induced cell death (AICD) of T-cells in
ITP
. Using a proliferation assay we found that CD3+ lymphocytes from
ITP
patients were significantly more resistant to dexamethasone induced suppression compared to normal lymphocytes. We also found that cultured CD3+ lymphocytes from
ITP
patients in remission were more susceptible to apoptosis both in the presence and absence of dexamethasone compared to cells from patient with active
ITP
and healthy controls, as indicated by increased staining of AnnexinV binding. Our findings suggest that apoptotic resistance of activated T-lymphocytes in patients with active
ITP
may lead to defective clearance of autoreactive T-lymphocytes through AICD, which might cause a continued immune destruction of platelets. Conversely, a loss of resistance to AICD in
ITP
patients in remission might be an important mechanism for the achievement of remission.
...
PMID:Disturbed apoptosis of T-cells in patients with active idiopathic thrombocytopenic purpura. 1563 May 4
