Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.60 (
caspase-7
)
920
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Recent studies have implicated the dying cell as a potential reservoir of modified autoantigens that might initiate and drive systemic autoimmunity in susceptible hosts. A number of subunits of the exosome, a complex of 3'-->5' exoribonucleases that functions in a variety of cellular processes, are recognized by the so-called anti-PM/Scl autoantibodies, found predominantly in patients suffering from an overlap syndrome of myositis and scleroderma. Here we show that one of these subunits,
PM/Scl-75
, is cleaved during apoptosis.
PM/Scl-75
cleavage is inhibited by several different caspase inhibitors. The analysis of
PM/Scl-75
cleavage by recombinant caspase proteins shows that
PM/Scl-75
is efficiently cleaved by caspase-1, to a smaller extent by caspase-8, and relatively inefficiently by caspase-3 and
caspase-7
. Cleavage of the
PM/Scl-75
protein occurs in the C-terminal part of the protein at Asp369 (IILD369 [see text] G), and at least a fraction of the resulting N-terminal fragments of
PM/Scl-75
remains associated with the exosome. Finally, the implications of
PM/Scl-75
cleavage for exosome function and the generation of anti-
PM/Scl-75
autoantibodies are discussed.
...
PMID:Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis. 1728 Jun 3
