Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.60 (
caspase-7
)
920
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
IGFBP-3
is known to possess intrinsic biological activities such as anti-tumor property in addition to its IGF/IGF-R axis-dependent actions in a variety of human cancers including breast cancer. To investigate the molecular mechanisms underlying the intrinsic biological actions of
IGFBP-3
on breast cancer cells, we performed yeast two-hybrid screening and found GRP78, known to cause drug-resistance, as a binding partner of
IGFBP-3
. Overexpression of
IGFBP-3
in antiestrogen-resistant LCC9 cells showed that
IGFBP-3
interacted with GRP78, resulting in disruption of the GRP78-
caspase-7
complex, thereby activating
caspase-7
, and further inducing apoptosis. Combination of overexpression of
IGFBP-3
and application of siRNAs against GRP78 led to decrease in cell viability upon ICI 182,780 treatment. These data suggest that
IGFBP-3
could sensitize antiestrogen-resistant breast cancer cells to ICI 182,780 by preventing the anti-apoptotic function of GRP78.
...
PMID:IGFBP-3 sensitizes antiestrogen-resistant breast cancer cells through interaction with GRP78. 2280 Dec 19
