Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.60 (
caspase-7
)
920
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Influenza A virus (IAV) exploits host factors to multiply and cause disease. An in-depth knowledge of this interaction of IAV with the host will aid the development of anti-IAV intervention strategies. Previously, we demonstrated that host
cortactin
, an actin filament-binding protein promotes IAV infection, but undergoes degradation via a lysosome-associated apoptotic pathway during the late stages of IAV infection. Next, we wanted to further understand the mechanisms and significance of this phenomenon. By using the RNA interference screens and site-directed mutagenesis followed by western blotting, we found that lysosome protease, cathepsin C is involved in
cortactin
degradation in human cells infected with IAV. Furthermore, executioner apoptotic caspase, caspase-3 not caspase-6 or
caspase-7
is involved in
cortactin
degradation during IAV infection, and caspase-3 cleavage site is located in the first actin-binding repeat of
cortactin
polypeptide. Finally, when expressed ectopically, the cleavage-resistant
cortactin
mutants decreased the amount of IAV progeny released from infected cells that was enhanced by the cleavage-sensitive
cortactin
wild type. These data strengthen the hypothesis proposed earlier that host
cortactin
plays an inhibitory role during the late stages of IAV infection, and IAV is facilitating its degradation to undermine such function.
...
PMID:Caspase-Mediated Cleavage of Human Cortactin during Influenza A Virus Infection Occurs in Its Actin-Binding Domains and Is Associated with Released Virus Titres. 3194 Sep 55
