Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.60 (
caspase-7
)
920
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Caspases are an important protease family that coordinate inflammation and programmed cell death. Two closely related caspases, caspase-3 and
caspase-7
, exhibit largely overlapping substrate specificities. Assessing their proteolytic activities individually has therefore proven extremely challenging. Here, we constructed an outer
membrane protein
G (OmpG) nanopore with a caspase substrate sequence DEVDG grafted into one of the OmpG loops. Cleavage of the substrate sequence in the nanopore by
caspase-7
generated a characteristic signal in the current recording of the OmpG nanopore that allowed the determination of the activity of
caspase-7
in Escherichia coli cell lysates. Our approach may provide a framework for the activity-based profiling of proteases that share highly similar substrate specificity spectrums.
...
PMID:A Nanopore Approach for Analysis of Caspase-7 Activity in Cell Lysates. 3142 65
