Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.6 (
chymopapain
)
407
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two forms of proteinase omega were isolated from a commercial preparation of
chymopapain
(
EC 3.4.22.6
) by means of cation-exchange liquid chromatography. Their circular dichroism (CD) spectra in the 182-320 nm region indicated that the two forms possess closely related structures. For comparison, we also recorded the CD spectra of chromatographically purified samples of papain (EC 3.422.2) and the most abundant form of
chymopapain
. According to the qualitative criteria proposed by Manavalan and Johnson (1983) Nature 305, 831-832), the spectral characteristics of papain correctly indicate that this protein belongs to the alpha + beta class.
Proteinase
omega is also placed in the alpha + beta category, while
chymopapain
seems to be an alpha/beta protein. Quantitative estimation of secondary structures yielded contents of helices and parallel beta-sheet that were higher in the case of
chymopapain
. Thus, the results of this work suggest that there are some differences in the folding pattern of
chymopapain
with respect to the other two proteinases. This proposal seems unexpected when the high amino acid sequence identity among these enzymes is considered.
...
PMID:Circular dichroism of cysteine proteinases from papaya latex. Evidence of differences in the folding of their polypeptide chains. 173 51
