Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.56 (
caspase-3
)
35,750
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The mechanisms governing tumorigenesis of gastric cancer have been an area of intense investigation. Currently, plant homeodomain (PHD) finger (PHF) proteins have been implicated in both tumor suppression and progression. However, the function of
PHF10
has not been well characterized. Here, we show that various levels of
PHF10
protein were observed in gastric cancer cell lines. Alteration of
PHF10
expression, which is associated with tumor cell growth, may result in apoptosis in gastric cancer cells both in vitro and in vivo. Knockdown of
PHF10
expression in gastric cancer cells led to significant induction of
caspase-3
expression at both the RNA and protein levels and thus induced alteration of
caspase-3
substrates in a time-dependent manner. Moreover, results from luciferase assays indicated that
PHF10
acted as a transcriptional repressor when the two PHD domains contained in
PHF10
were intact. Combined with previous findings, our data suggest that
PHF10
transcriptionally regulates the expression of
caspase-3
. Finally, by using systematic reporter deletion and chromatin immunoprecipitation assays, we localized a region between nucleotides -270 and -170 in the
caspase-3
promoter that was required for the efficient inhibition of
caspase-3
promoter activity by
PHF10
. Collectively, our findings show that
PHF10
repressed
caspase-3
expression and impaired the programmed cell death pathway in human gastric cancer at the transcriptional level.
...
PMID:A novel plant homeodomain finger 10-mediated antiapoptotic mechanism involving repression of caspase-3 in gastric cancer cells. 2053 Jul 14
