Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.22.54 (calpain 3)
 430 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) p10 gene region was cloned, sequenced and the putative p10 gene expression characterized by Northern-blot analysis. Sequence analysis of the p10 gene region indicated the presence of two complete open reading frames (ORFs) of 713 and 281 nucleotides, which codes for polypeptides of 273 and 93 amino acids, with homology to the P26 and P10 proteins of baculoviruses, respectively. Two additional partial ORFs, coding for partial polypeptides of 110 and 146 amino acids, showed homology to the p22.2 gene of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) and p74 genes of different baculoviruses, respectively. A small ORF of 224 nucleotides coding for a protein of 74 amino acids showed homology to the 3'-end of the early p94 gene of AcMNPV. A putative baculovirus very late promoter motif TAAG was identified in the 5'-non-translated region (5'-UTR) at position-54 upstream of the start codon. The consensus polyadenylation sequence AATAAA is present 146nt downstream of the termination codon and the p10 ORF is flanked by the p26 and p74 ORFs. Homology comparisons showed that the P10 protein of AgMNPV is most closely related (82% amino acid sequence identity) to the P10 from the Orgyia pseudotsugata nucleopolyhedrovirus (OpMNPV). Transcriptional analysis of the AgMNPV p10 gene showed that p10-specific transcripts could be detected late in infection.
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PMID:Characterization of the p10 gene region of Anticarsia gemmatalis nucleopolyhedrovirus. 1208 45

The genomic RNA (gRNA) of Pea enation mosaic virus 2 (PEMV2) is the template for p33 and -1 frameshift product p94. The PEMV2 subgenomic RNA (sgRNA) encodes two overlapping ORFs, p26 and p27, which are required for movement and stability of the gRNA. Efficient translation of p33 requires two of three 3' proximal cap-independent translation enhancers (3'CITEs): the kl-TSS, which binds ribosomes and engages in a long-distance interaction with the 5'end; and the adjacent eIF4E-binding PTE. Unlike the gRNA, all three 3'CITEs were required for efficient translation of the sgRNA, which included the ribosome-binding 3'TSS. A hairpin in the 5' proximal coding region of p26/p27 supported translation by the 3'CITEs by engaging in a long-distance RNA:RNA interaction with the kl-TSS. These results strongly suggest that the 5' ends of PEMV2 gRNA and sgRNA connect with the 3'UTR through similar long-distance interactions while having different requirements for 3'CITEs.
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PMID:Differential use of 3'CITEs by the subgenomic RNA of Pea enation mosaic virus 2. 2875 Mar 23