Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.36 (
caspase-1
)
6,285
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Intracellular trafficking is a tightly regulated cellular process, mediated in part by Rab GTPases and their corresponding effector proteins. Viruses have evolved mechanisms to hijack these processes to promote their lifecycles. Here we describe a mechanism by which cleavage of the Rab7 adaptor protein, RILP (
Rab interacting lysosomal protein
) is induced by viral infection. We report that RILP is directly cleaved by
caspase-1
and we have identified a novel
caspase-1
recognition site at aspartic acid 75 within the RILP sequence. Alanine substitution at D75 blocks
caspase-1
-mediated RILP cleavage. Full-length RILP localizes in a tight vesicular structure near the perinuclear region while the cleaved form of RILP re-distributes throughout the cytoplasm. However, cleavage alone was insufficient to re-localize RILP to the cellular periphery and re-localization required specific phosphorylation events near the
caspase-1
recognition site. The combination of cleavage and phosphorylation were both needed for release from the dynein component p150
Glued
and redistribution of CD63
+ve
intracellular vesicles.
...
PMID:Caspase-1 regulates cellular trafficking via cleavage of the Rab7 adaptor protein RILP. 3010 68
