Gene/Protein Disease Symptom Drug Enzyme Compound
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 6,285 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A method for the quantitative determination of 11 organic acids (oxalic acid, citric acid, tartaric acid, malic acid, ascorbic acid, lactic acid, succinic acid, formic acid, acetic acid, glutaric acid and fumaric acid) in fruit juice was developed successfully. It was based on an ion exclusion chromatographic separation under the conditions of isocratic elution with 17 mmol/L sulphuric acid solution with the ICE-ION-300 ion exclusion column, and the UV detection at 210 nm. The precision of the method was investigated and the relative standard deviations were from 1.5% to 9.8% (n = 10).
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PMID:[Simultaneous determination of 11 organic acids in fruit juice by ion exclusion chromatography]. 1254 18

Acrylamide, a known neurotoxin and putative human carcinogen, has been included among the substances to be monitored in drinking water according to the European Union Directive 98/83 on potable water. This paper reports a new method based on the combination of ion-exclusion chromatographic separation and MS detection. Samples of drinking water have been directly injected in the microbore ICE-AS1 column and detected in the selected-ion monitoring mode by a single quadrupole system with electrospray ionization. Chromatographic conditions, such as eluent composition and flow rate, have been optimized by a central composite design experiment. Statistical analysis of data showed that the amount of acetonitrile fraction in the eluent mixture, composed by acetonitrile and formic acid solution, is the variable that most influences retention of the acrylamide peak. After optimization of MS detection parameters, this method has been validated for spiked drinking water samples. The effect of large-volume injection (up to 500 microl) has been also explored. Linearity was evaluated from 0.5 to 5 microg l(-1). Repeatability, expressed as R.S.D., was 16 and 12% at 0.5 and 1 microg l(-1) respectively. The limit of detection was 0.20 ppb with 500 microl injection volume.
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PMID:Determination of acrylamide in drinking water by large-volume direct injection and ion-exclusion chromatography-mass spectrometry. 1525 Apr 18

A simple and sensitive liquid chromatographic-tandem mass spectrometric (LC/MS/MS) method has been developed and validated to confirm and quantify acrylamide monomer (AA) in drinking water using [13C3] acrylamide as internal standard (IS). After a preconcentration by solid-phase extraction with spherical activated carbon, analytes were chromatographed on IonPac ICE-AS1 column (9 x 250 mm) under isocratic conditions using acetonitrile-water-0.1 M formic acid (43 + 52 + 5, v/v/v) as the mobile phase. Analysis was achieved using a triple-quadrupole mass analyzer equipped with a turbo ion spray interface. For confirmation and quantification of the analytes, MS data acquisition was performed in the multireaction monitoring mode, selecting 2 precursor ion to product ion transitions for both AA and IS. The method was validated for linearity, sensitivity, accuracy, precision, extraction efficiency, and matrix effect. Linearity in tap water was observed over the concentration range 0.1-2.0 microg/L. Limits of detection and quantification were 0.02 and 0.1 microg/L, respectively. Interday and intraday assays were performed across 3 validation levels (0.1, 0.5, and 1.5 microg/L). Accuracy (as mean recovery) ranged from 89.3 to 96.2% with relative standard deviation <7.98%. Performance characteristics of this LC/MS/MS method make it suitable for regulatory confirmatory analysis of AA in drinking water in compliance with European Union and U.S. Environmental Protection Agency standards.
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PMID:Determination of low-level acrylamide in drinking water by liquid chromatography/tandem mass spectrometry. 1938 85

A new method was developed for the determination of maleic hydrazide (MH) in potatoes, onions and garlics by high performance ion-exclusion chromatography (HPIEC). The sample was extracted with acidic methanol, and then analyzed by HPIEC. The analytical column was IonPac ICE-AS1 (250 mm x 9 mm) and a mixture of 3 mmol/L formic acid water solution-acetonitrile (70: 30, v/v) was used as the eluent at a flow rate of 0.8 mL/min. The detection was performed at 205 nm by an Ultimate 3000 VWD. The quantitative analysis of external standard calibration curves was used. The linear range of the method for MH was 0.006 - 1.0 mg/L (r = 0.999 9). The average recoveries were 91% - 103% with relative standard deviations (RSDs) less than 3%. The detection limit was 0.002 mg/L for MH. The method is simple, effective, precise, sensitive, reproducible and selective. It can be used to determine the residue of MH in potatoes, onions and garlics.
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PMID:[Determination of maleic hydrazide in vegetables by high performance ion-exclusion chromatography]. 2104 93