Gene/Protein Disease Symptom Drug Enzyme Compound
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Biological processes, geochemical reactions, anthropogenic emissions, and transformation reactions of xenobiotics are responsible for the widespread occurrence of aliphatic carboxylic acids in the environment. To study the performance of the ion-exclusion chromatography column IonPac ICE-AS6 in the analysis of environmental and environmental-technical samples, organic acids are investigated in composting seepage, silage effluents, aqueous extracts of sewage sludge, molasses hydrolysate, and alkaline cellulose hydrolysates. With respect to the diverse sample matrix and composition, different chromatographic conditions are applied. It is possible to determine various volatile fatty acids, dicarboxylic acids, (poly)hydroxy acids, and keto acids as main and trace components in samples with very high and low dissolved organic carbon content. Low baseline noise allows the determination of malic and succinic acid in the concentration range of approximately 1 microM/L in the presence of higher concentrations of fully ionized compounds. The applicability of the column in environmental analysis may be limited by the poor retardation of strong organic acids, insufficient separation of some relevant substance combinations (i.e., citric and isocitric acid), and very strong hydrophobic interactions with straight-chain monocarboxylic acids containing four or more carbon atoms.
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PMID:Chromatographic properties of the ion-exclusion column IonPac ICE-AS6 and its application in environmental analysis, Part II: application in environmental analysis 1101 25

A method for the quantitative determination of 11 organic acids (oxalic acid, citric acid, tartaric acid, malic acid, ascorbic acid, lactic acid, succinic acid, formic acid, acetic acid, glutaric acid and fumaric acid) in fruit juice was developed successfully. It was based on an ion exclusion chromatographic separation under the conditions of isocratic elution with 17 mmol/L sulphuric acid solution with the ICE-ION-300 ion exclusion column, and the UV detection at 210 nm. The precision of the method was investigated and the relative standard deviations were from 1.5% to 9.8% (n = 10).
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PMID:[Simultaneous determination of 11 organic acids in fruit juice by ion exclusion chromatography]. 1254 18