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Query: EC:3.4.22.36 (
caspase-1
)
6,285
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The aim of this study was to unravel the genetic determinants responsible for multidrug (including carbapenems) resistance and virulence in a clinical isolate of
Klebsiella quasipneumoniae
subsp.
similipneumoniae
by whole-genome sequencing and comparative analyses. Eighty-three clinical isolates initially identified as carbapenem-resistant
K. pneumoniae
were collected from nosocomial infections in southeast Brazil. After RAPD screening, the
KPC
-142 isolate, showing the most divergent DNA pattern, was selected for complete genome sequencing in an Illumina HiSeq 2500 instrument. Reads were assembled into scaffolds, gaps between scaffolds were resolved by
in silico
gap filling and extensive bioinformatics analyses were performed, using multiple comparative analysis tools and databases. Genome sequencing allowed to correct the classification of the
KPC
-142 isolate as
K. quasipneumoniae
subsp.
similipneumoniae
. To the best of our knowledge this is the first complete genome reported to date of a clinical isolate of this subspecies harboring both class A beta-lactamases
KPC
-2 and OKP-B-6 from South America.
KPC
-142 has one 5.2 Mbp chromosome (57.8% G+C) and two plasmids: 190 Kbp
p
KQPS142a (50.7% G+C) and 11 Kbp
p
KQPS142b (57.3% G+C). The 3 Kbp region in
p
KQPS142b containing the
bla
KPC
-2
was found highly similar to that of
p
Kp13d of
K. pneumoniae
Kp13 isolated in Southern Brazil in 2009, suggesting the horizontal transfer of this resistance gene between different species of
Klebsiella
.
KPC
-142 additionally harbors an integrative conjugative element
ICE
Pm1
that could be involved in the mobilization of
p
KQPS142b and determinants of resistance to other classes of antimicrobials, including aminoglycoside and silver. We present the completely assembled genome sequence of a clinical isolate of
K. quasipneumoniae
subsp.
similipneumoniae
, a
KPC
-2 and OKP-B-6 beta-lactamases producer and discuss the most relevant genomic features of this important resistant pathogen in comparison to several strains belonging to
K. quasipneumoniae
subsp.
similipneumoniae
(phylogroup II-B),
K. quasipneumoniae
subsp.
quasipneumoniae
(phylogroup II-A),
K. pneumoniae
(phylogroup I), and
K. variicola
(phylogroup III). Our study contributes to the description of the characteristics of a novel
K. quasipneumoniae
subsp.
similipneumoniae
strain circulating in South America that currently represent a serious potential risk for nosocomial settings.
...
PMID:Comparative Genomic Analysis of a Clinical Isolate of
Klebsiella quasipneumoniae
subsp.
similipneumoniae
, a KPC-2 and OKP-B-6 Beta-Lactamases Producer Harboring Two Drug-Resistance Plasmids from Southeast Brazil. 2950 35
