Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.22.36 (caspase-1)
 6,285 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

High-performance ion chromatography (HPIC) has been utilised to probe the biochemistry associated with changes in tumour pH following total vascular occlusion. Samples from the tumour extracellular compartment were obtained by insertion of a microdialysis probe and analysed by HPIC with conductivity detection. Separations were carried out by ion-exclusion chromatography using an IonPac ICE AS1 weak-acid column. The eluent (0.5 mM octanesulphonic acid) was chemically suppressed with 5 mM tetrabutylammonium hydroxide through a micromembrane suppressor. After complete vascular occlusion induced by a clamp, lactate levels increased in the extracellular compartment.
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PMID:Analysis of the acidic microenvironment in murine tumours by high-performance ion chromatography. 764 Jul 78

Acrylamide, a known neurotoxin and putative human carcinogen, has been included among the substances to be monitored in drinking water according to the European Union Directive 98/83 on potable water. This paper reports a new method based on the combination of ion-exclusion chromatographic separation and MS detection. Samples of drinking water have been directly injected in the microbore ICE-AS1 column and detected in the selected-ion monitoring mode by a single quadrupole system with electrospray ionization. Chromatographic conditions, such as eluent composition and flow rate, have been optimized by a central composite design experiment. Statistical analysis of data showed that the amount of acetonitrile fraction in the eluent mixture, composed by acetonitrile and formic acid solution, is the variable that most influences retention of the acrylamide peak. After optimization of MS detection parameters, this method has been validated for spiked drinking water samples. The effect of large-volume injection (up to 500 microl) has been also explored. Linearity was evaluated from 0.5 to 5 microg l(-1). Repeatability, expressed as R.S.D., was 16 and 12% at 0.5 and 1 microg l(-1) respectively. The limit of detection was 0.20 ppb with 500 microl injection volume.
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PMID:Determination of acrylamide in drinking water by large-volume direct injection and ion-exclusion chromatography-mass spectrometry. 1525 Apr 18

A coulometric analysis method and an ion-exclusion chromatographic method were developed for the determination of antimony(V) in a large excess of antimony(III). Antimony(V) reacted with potassium iodide in a high concentration hydrochloric acid; the liberated iodine was determined by the standard-addition method using coulometrically generated iodine. Using a Dionex ICE-AS1 ion-exclusion column, antimony(V) was eluted with 40 mmol/L sulfuric acid; on the other hand, antimony(III) was strongly retained on the column. The content, expressed as the amount ratio of antimony(V) to antimony(III), was 0.035% in a 10 g/kg antimony(III) solution prepared from an antimony(III) oxide reagent by the coulometric analysis method and 0.036% in a 1 g/kg antimony(III) solution prepared from the same antimony(III) oxide by the ion-exclusion chromatographic method. The results of both methods were in good agreement with each other. The detection limit of antimony(V) in antimony(III) oxide by the former method was 0.004% of antimony(III), and that by the latter method was 0.002% of antimony(III).
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PMID:A coulometric analysis method and an ion-exclusion chromatographic method for the determination of antimony(V) in large excess of antimony(III). 1878 Oct 19

A simple and sensitive liquid chromatographic-tandem mass spectrometric (LC/MS/MS) method has been developed and validated to confirm and quantify acrylamide monomer (AA) in drinking water using [13C3] acrylamide as internal standard (IS). After a preconcentration by solid-phase extraction with spherical activated carbon, analytes were chromatographed on IonPac ICE-AS1 column (9 x 250 mm) under isocratic conditions using acetonitrile-water-0.1 M formic acid (43 + 52 + 5, v/v/v) as the mobile phase. Analysis was achieved using a triple-quadrupole mass analyzer equipped with a turbo ion spray interface. For confirmation and quantification of the analytes, MS data acquisition was performed in the multireaction monitoring mode, selecting 2 precursor ion to product ion transitions for both AA and IS. The method was validated for linearity, sensitivity, accuracy, precision, extraction efficiency, and matrix effect. Linearity in tap water was observed over the concentration range 0.1-2.0 microg/L. Limits of detection and quantification were 0.02 and 0.1 microg/L, respectively. Interday and intraday assays were performed across 3 validation levels (0.1, 0.5, and 1.5 microg/L). Accuracy (as mean recovery) ranged from 89.3 to 96.2% with relative standard deviation <7.98%. Performance characteristics of this LC/MS/MS method make it suitable for regulatory confirmatory analysis of AA in drinking water in compliance with European Union and U.S. Environmental Protection Agency standards.
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PMID:Determination of low-level acrylamide in drinking water by liquid chromatography/tandem mass spectrometry. 1938 85

A new method was developed for the determination of maleic hydrazide (MH) in potatoes, onions and garlics by high performance ion-exclusion chromatography (HPIEC). The sample was extracted with acidic methanol, and then analyzed by HPIEC. The analytical column was IonPac ICE-AS1 (250 mm x 9 mm) and a mixture of 3 mmol/L formic acid water solution-acetonitrile (70: 30, v/v) was used as the eluent at a flow rate of 0.8 mL/min. The detection was performed at 205 nm by an Ultimate 3000 VWD. The quantitative analysis of external standard calibration curves was used. The linear range of the method for MH was 0.006 - 1.0 mg/L (r = 0.999 9). The average recoveries were 91% - 103% with relative standard deviations (RSDs) less than 3%. The detection limit was 0.002 mg/L for MH. The method is simple, effective, precise, sensitive, reproducible and selective. It can be used to determine the residue of MH in potatoes, onions and garlics.
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PMID:[Determination of maleic hydrazide in vegetables by high performance ion-exclusion chromatography]. 2104 93