Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.22.32 (
bromelain
)
1,025
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The serine proteinases trypsin, chymotrypsin, elastase, and
acrosin
bind to the proflavin resin, the sulfhydryl proteinases ficin,
bromelain
, and papain are retarded by the resin, whereas most proteins and enzymes tested are not bound. Elution of the bound activities is accomplished NaCl or by variation from the pH optimum of the enzyme. Commercially available enzymes that are bound or retarded are easily further purified by the column. The
acrosin
activity of sperm acrosomal extracts is separated into bound and unbound activities.
Acrosin
is purified 120-fold from sperm acrosomal extracts in a single step, yielding a specific activity of 96.
...
PMID:Fractionation of of proteolytic enzymes by affinity chromatography on sepharose aminocaproyl proflavin. 100 11
