Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.9 (
enterokinase
)
675
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Fusion gene consisting of dextran-binding domain from Leuconostoc mesenteroides subsp. Mesenteroides (DBD) and human recombinant
interferon-beta
(
IFN-beta
) incorporated between the nucleotide sequence encoding for the recognition site of human
enteropeptidase
(DDDDK) was installed and constructed in Escherichia coli. The overproducing strain of the chimeric protein DBD-
IFN-beta
consisting of the
IFN-beta
, spacer including 10 GS-repeats, human
enteropeptidase
recognition site, and dextran-binding domain from Leuconostoc mesenteroides was constructed. Free human recombinant
interferon-beta
was obtained as a result of treatment of the chimeric protein DBD-
IFN-beta
immobilized on sephadex G-25 with human
enteropeptidase
. The ability of free and immobilized protein to protect human cells from viral infection was demonstrated. The developed approach can be used for purification of the recombinant proteins with different biological activity and possible construction of new immunostimulating and antiviral drugs, growth factors, anti-cancer drugs, etc.
...
PMID:[Human recombinant interferon-B constructed on the basis of affinity-binding domain technology]. 2001 62
