Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.9 (
enterokinase
)
675
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The extracellular loop 2 of a tetraspanin from Schistosoma japonicum (Sj-
TSP
-2) is homologous to Schistosoma mansoni
TSP
-2. In our initial study, Sj-
TSP
-2 is an identical antigen against schistosomiasis caused by S. japonicum. Through the pET32 vector system and nickel (Ni)-absorbed chelating Sepharose, Sj-
TSP
-2 was expressed and purified as a soluble fusion constructed with an N-terminal thioredoxin-His(6)-EK protease site tag (Trx-
TSP
-2). In phosphate buffer (PB) with a low concentration of imidazole, the Trx-
TSP
-2 fusion protein was efficiently cleaved by
enterokinase
(EK). Sj-
TSP
-2 was isolated and enriched using cobalt (Co)-absorbed chelating Sepharose and HiTrap SP column. Character of the protein was analyzed via animal experiments and then clinical trials. The purification approach yielded pure Sj-
TSP
-2, which will provide feasible advices for discovering vaccines against schistosomiasis.
...
PMID:Schistosoma japonicum: efficient and rapid purification of the tetraspanin extracellular loop 2, a potential protective antigen against schistosomiasis in mammalian. 2068 2
