Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.73 (urokinase-type plasminogen activator)
 10,685 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Adherence ability to catheter materials for intravenous use of coagulase-negative staphylococci (CNS) which is frequently isolated from prosthetic implants and medical devices inserted into the body was studied, and the mechanism of catheter-associated infections and the prevention against that were discussed. Difference of the adherence ability of CNS to various kinds of catheter materials was found, and adherence ability to the catheter materials was correlated with that of human epithelial cells in culture. CNS isolated from intravenous catheters had higher adherence ability to the human epithelial cell cultures than isolates from other clinical sources. Among 4 kinds of catheter materials, including ethylene vinyl acetate (EVA), polyvinyl chloride (PVC), silicone (Sil) and polyurethane (PUR), the adherence ability of CNS on the EVA containing barium was highest, and Sil treated with tungsten showed the lowest. The physicochemical properties of both the bacteria and the catheter materials, hydrophobicity of the bacterial cultures and the catheter materials, negative charges on surface of the bacteria, surface structure of the catheter materials, additives to the catheter materials, and coating the catheter surface gave great effects on the adherence of CNS to the catheter materials. In addition, as biofactors, fibronectin and PROTEAMIN-HICALIQ solutions enhanced the adherence of CNS. These results suggested that catheter materials having a smooth surface and higher hydrophobicity, treated with tungsten and coated with urokinase were effective on the suppression of the adherence to catheter materials. In addition to the improvement of catheter materials, aseptic procedures in catheterization were thought to be critical for the prevention of catheter-associated infections.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:[Adherence ability of coagulase-negative staphylococci to catheter materials]. 207 72

The lung plasminogen activator (PA) response was examined in four different models of particle-induced pulmonary lesions in NMRI mice (single intratracheal administration, 0.75 to 5 mg/mouse). Sequential changes in cellular (total and differential counts) and biochemical markers of alveolitis (lactate dehydrogenase [LDH], total proteins) were monitored in bronchoalveolar fluid (BALF) and the fibrotic lung response was assessed histologically. An intense but spontaneously resolving alveolitis was produced by manganese dioxide (MnO2) and a fibrosing alveolitis was elicited by crystalline silica (DQ12). Minimal and noninflammatory responses were obtained after instillation of titanium dioxide (TiO2) and tungsten carbide (WC), respectively. The comparison between the resolving and the fibrosing alveolitis model was especially taken into consideration in an attempt to identify fibrinolytic changes associated with the development of fibrosis. At the alveolitis stage, similarly increased BALF PA activities were measured in both the resolving and the fibrosing alveolitis models whereas only slight and no PA modifications were noted after administration of TiO2 and WC, respectively. Persistently (up to 120 d) increased BALF PA activity was selectively associated with the progression to fibrosis (DQ12), suggesting that PA is involved in the fibrotic process. ELISA measurements demonstrated that the changes in BALF PA activity were exclusively related to changes in urokinase (uPA), not tissue-type PA. A rapid and persisting (up to Day 30) upregulation of cell-associated PA activity occurred after DQ12, MnO2, and TiO2 treatment only. Cellular PA activity was however significantly higher in fibrogenic inflammatory cells recovered from DQ12 than from MnO2-treated mice suggesting that the intensity of cellular PA upregulation may represent an early indicator of the progression to fibrosis. The implication of urokinase in the pathogenesis of silica-induced fibrosis was demonstrated by the use of a uPA knockout mice. The acceleration of the fibrotic process in uPA-deficient compared with the wild type animals demonstrated the contribution of uPA to limit the fibrotic process.
 ...
PMID:Role of urokinase in the fibrogenic response of the lung to mineral particles. 947 81