Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.7 (plasmin)
 9,023 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Blood brain cell contact activates lipid peroxidation and arachidonic acid metabolism as shown in animal experiments. Previous studies in tumor patients have shown that enhanced cysteinyl-leukotriene (cys-LT) formation leads to increasing perifocal edema. This suggests their involvement in CNS pathology. The purpose of this study is to measure the amounts of cys-LT released during blood-brain cell contact in patients with spontaneous intracerebral hemorrhage (ICH). Seventeen patients were divided into two groups. One of them was treated conservatively, the other group received a local rTPA therapy after stereotactic puncture and hematoma reduction by aspiration. Before treatment as well as during the following 5 days cerebral cys-LT release was measured analyzing the metabolites in patients urine. The mean cys-LT release before treatment ranged at 14.51 +/- 1.13 pg/mg creatinine/ml hematoma volume. In the conservative treatment group urinary cys-LT release dropped to 14.05 +/- 1.1 pg/mg creatinine/ml hematoma volume by the fifth day of treatment. The change of urinary cys-LT release in the operatively treated patients was much more distinct: Five days after rTPA therapy urinary cys-LT release was 11.23 +/- 0.6 pg/mg creatinine/ml tumor volume. The urinary cys-LT excretion at the end of the measurement was significantly lower in the operatively treated group (p < 0.05). In an additional experimental approach using dissociated rat brain cells plasmin was excluded as an activator for cerebral cys-LT formation. Variation in incubation time as well as the concentration of plasmin exhibited no difference in cys-LT release in comparison to the incubation of dissociated rat brain cells without any external stimulus. These results emphasize that rTPA does not influence cys-LT formation in a direct way. After experimental evidence, these results indicate now in a clinical evaluation that cerebral cys-LT formation is activated during blood-brain cell contact also in patients suffering from intracerebral hemorrhage. The amounts of cys-LT release depend on hematoma volume. Hematoma reduction by aspiration and rTPA treatment is followed by a distinct reduction of cys-LT release.
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PMID:[Leukotriene synthesis after intracerebral hemorrhage: a further indicator for their pathophysiologic significance in the CNS]. 967 1

In 12 operatively (stereotactic aspiration and recombinant tissue-type plasminogen activator, rTPA) and 5 conservatively treated patients with spontaneous intracerebral hemorrhage the amounts of cysteinyl-leukotriene (cys-LT) released by blood-brain cell contact were measured by the urinary excretion of their metabolites during treatment. The mean cys-LT release before treatment was 14.51 +/- 1.13 pg/mg creatinine/ml hematoma volume. The urinary cys-LT excretion at the end of the measurements was significantly lower in the operatively treated group than in the patients with conservative therapy (p < 0.05). We also found a significant correlation between the perifocal edema volume and the amount of cys-LT measured in patients' urine (p < 0.01). In an additional animal experiment using dissociated rat brain cells plasmin was excluded as an activator for cerebral cys-LT formation, which emphasizes that rTPA did not influence cys-LT formation.
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PMID:Cysteinyl-leukotriene levels in intracerebral hemorrhage: an edema-promoting factor? 977 48