Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.21.7 (plasmin)
 9,023 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

As a first step in our study of structure-function relationships among primate and non-primate growth hormones, human growth hormone (hGH) was subjected to the limited digestive activity of human plasmin. The lyophilized whole digest, containing less than 2% of unchanged hormone, had an average of 2.3 new amino-terminal groups per mole. The digest had the same potency as the native hormone (a) in causing weight gain in hypophysectomized rats; (b) in stimulating somatomedin production in hypophysectomized rats; (c) in stimulating upake of [(3)H]leucine into isolated diaphragm of hypophysectomized rats; (d) in accelerating transport of [(14)C]alpha-aminoisobutyric acid into isolated diaphragm of hypophysectomized rats; (e) in stimulating uptake of [3-0-methyl-(14)C]glucose by isolated adipose tissue of hypophysectomized rats; (f) in accelerating conversion of [(14)C]glucose to (14)CO(2) by isolated epididymal adipose tissue of hypophysectomized rats. The digest also caused glucosuria in partially pancreatectomized rats treated with dexamethasone. These metabolic actions of plasmin-digested hGH in the array of animal tests were confirmed by comparable effects elicited in 11 human subjects (nine pituitary-deficient children and adolescents and two nondeficient adults). A single injection of the plasmin digest caused an increase in plasma free fatty acids and a fall in plasma amino acids. Seven daily injections caused positive balances of nitrogen, phosphorous, sodium, and potassium, gain in body weight, and in two of three subjects impairment of glucose tolerance. The potency of the plasmin digest in producing these metabolic effects in man was comparable to that of native hGH.Thus, 2-3 bonds in the hGH molecule can be cleaved by plasmin without impairing the hormone's growthpromoting, anabolic, diabetogenic, and adipokinetic actions for rat and man.
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PMID:Metabolic effects of plasmin digests of human growth hormone in the rat and man. 427 Jun 45

A highly purified preparation of human GH (hGH) and two large fragment complexes (Da1 and Dc2) isolated from plasmin digests of reduced and S-carbamidomethylated hGH were tested for their ability to produce five different biological responses in segments of epididymal fat obtained from hypophysectomized rats. Judging by the minimal concentration of hormone needed to produce a statistically significant response, hGH was 3-10 times as potent as Da1 and Dc2 in increasing the oxidation of [U-14C]glucose to 14CO2, hGH and Da1 were equipotent in stimulating the oxidation of L-[1-14C]leucine to 14CO2, antagonizing the lipolytic effect of epinephrine, and inducing refractoriness to the insulin-like action of hGH. At least 3 times as much Dc2 was required to produce significant responses. Da1 was 10 times more active than hGH in producing delayed lipolysis in the presence of theophylline and 30 times as effective as Dc2. These findings suggest that the various responses to GH that can be measured in adipose tissue may result from more than one kind of interaction between GH and adipocytes. (Endocrinology 108: 553, 1981)
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PMID:Altered profiles of biological activity of growth hormone fragments on adipocyte metabolism. 677 84