Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.68 (
tissue plasminogen activator
)
11,311
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Human tissue plasminogen activator (t-PA) was shown to bind specifically to human osteosarcoma cells (HOS), and human epidermoid carcinoma cells (A-431 cells). Crosslinking studies with DTSSP demonstrated high molecular weight complexes (130,000) between 125I-
t-PA
and cell membrane protein on human umbilical vein endothelial cells (HUVEC), HOS, and A-431 cells. A 48-65,000 molecular weight complex was demonstrated after crosslinking
t-PA
peptide (res. 7-20) to cells. Ligand blotting of cell lysates which had been passed over a
t-PA
affinity column revealed binding of
t-PA
to 54,000 and 95,000 molecular weight proteins. Several
t-PA
binding proteins were identified in immunopurified cell lysates, including
tubulin beta chain
, plasminogen activator inhibitor type 1 and single chain urokinase.
...
PMID:Characterization of tissue plasminogen activator binding proteins isolated from endothelial cells and other cell types. 212 40
