Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.64 (
proteinase K
)
4,071
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Scrapie is a prion (PrP
Sc
) disease of sheep. The incubation period of sheep scrapie is strongly influenced by polymorphisms at positions 136, 154, and 171 of a sheep's normal cellular prion protein (PrP
C
). Chymotrypsin was used to digest sheep recombinant PrP to identify a set of characteristic peptides [M
132
LGSXMSRPL
141
(X = A or V), Y
153
XENMY
158
(X,= H or R), and Y
166
RPVDXY
172
(X = H, K, Q, or R)] that could be used to detect and quantitate polymorphisms at positions 136, 154, and 171 of sheep PrP
C
or PrP
Sc
. These peptides were used to develop a multiple reaction monitoring method (MRM) to detect the amounts of a particular polymorphism in a sample of PrP
Sc
isolated from sheep heterozygous for their PrP
C
proteins. The limit of detection for these peptides was less than 50 attomole. Spinal cord tissue from heterozygous (ARQ/VRQ or
ARH
/ARQ) scrapie-infected Rasa Aragonesa sheep was analyzed using this MRM method. Both sets of heterozygotes show the presence of both polymorphisms in PrP
Sc
. This was true for samples containing both
proteinase K
(PK)-sensitive and PK-resistant PrP
Sc
and samples containing only the PK-resistant PrP
Sc
. These results show that heterozygous animals contain PrP
Sc
that is composed of significant amounts of both PrP polymorphisms.
...
PMID:Quantitating PrP Polymorphisms Present in Prions from Heterozygous Scrapie-Infected Sheep. 2793 97
