Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.64 (proteinase K)
 4,071 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Dig-labeled recombinant DNA probe of L. alstoni which contains the entire structural OmpL1 gene was hybridized with the recombinant DNA of the plasmid named pDJH2 of the gene library of L. interrogans serovar lai strain 017. The result showed a high degree of homology among them; expression of recombinant DNA of pDJH2 was achieved by beta-D-galactosidase (IPTG) induction in E. coli. The molecular weight of this product is 68kd. Then they were treated with proteinase K and subjected to SDS-PAGE. The results showed it is a protein in nature. Using the specific monoclonal antibody E4B7D5 on immunoblotting and specific polyclonal antibody on dot-ELISA assay, we investigated the immune reaction and noticed that protein 68kd might be an antigen in character. E. coli which contains the recombinant plasmid pDJH2 were injected into BALB/c mice. Then the mice were challenged by leptospires of the strong virulence strain 017, but all the infected mice survived. In this paper, we first report the expression of recombinant DNA of L. interrogans serovar lai strain 017 in E. coli when injected with IPTG, and immunoprotection of BALB/c mice which were injected with the expression against the infection of L. interrogans serovar lai strain 017. pDJH2 may be the first recombinant for which the gene has been cloned and its expression product 68kd may be the immunoprotective antigens.
Hua Xi Yi Ke Da Xue Xue Bao 1995 Sep
PMID:[Homology of the recombinant DNA of plasmid pDJH2 with the recombinant DNA probe of L. alstoni and analysis of its expression in Escherichia coli]. 858 84

P19 EC cells can be induced to differentiate into neurons in vitro when they were treated with 1x10(-6) mol/L retinoic acid. In the process of neuronal differentiation, P19 cells gradually migrate and form aggregates. Aggregated cells weakly adhered to the surface of cell culture dishes are usually dropped of during the procedure of in situ hybridization. To keep cells on the coverslips, we modified the procedure by using 1 g/L gelatin solution to cover the cells and substituting proteinase K treatment with pepsin treatment which digested cellular proteins mildly. With the treatment of pepsin, RNA probe can easily go through cell membrane and bind to its target, without loss of cell from coverslips.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1999
PMID:In Situ Hybridization of RA Induced P19 Cells. 1214 28