Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.64 (
proteinase K
)
4,071
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ehrlich ascites tumor cell putative nuclear pre-messenger RNA (pre-mRNA) was isolated under conditions minimizing RNA degradation by ribonucleases, aggregation, and non-specific protein-RNA interaction. Isolated under these conditions, it sedimented 10 to 12 S;
proteinase K
, a powerful proteolytic enzyme with a broad action spectrum, gave similar sedimentation values and polyacrylamide gel electrophoresis revealed the major component migrating ahead of 16S E. Coli rRNA marker.
Cesium chloride
buoyant density analysis of pre-mRNA revealed 2 components (1.51 and 1.68 g/cm3). Therefore, pre-mRNA appeared to be smaller than some previous reports.
...
PMID:Characterization of putative nuclear pre-messenger RNA of Ehrlich ascites tumor cells. 124 Dec 93
