Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.64 (
proteinase K
)
4,071
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Weakly beta-haemolytic human intestinal spirochaetes (w beta
HIS
) isolated in Italy showed an extrachromosomal band migrating at 4.3 kb after agarose gel electrophoresis of total DNA. The band was observed within 4 strains (HRM2, HRM4, HRM7 and HRM14) among the 7 w beta
HIS
analysed and was resistant to
proteinase K
and RNase treatment, whereas after purification it was completely digested by incubation with DNaseI. The origin, the structure and the significance of this extrachromosomal DNA are at present unknown. In addition to w beta
HIS
, the reference strain P18A of Serpulina hyodysenteriae was comparatively analysed and a 6.5 Kb extrachromosomal DNA element was found, as expected. This finding proves that the experimental conditions we used while searching for extrachromosomal DNA within w beta
HIS
were appropriate. To the best of our knowledge, this is the first report on the detection of extrachromosomal DNA from w beta
HIS
.
...
PMID:Detection of extrachromosomal DNA in Italian isolates of weakly beta-haemolytic human intestinal spirochaetes. 969 3
