Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.6 (
thromboplastin
)
13,278
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In order to elucidate the two-dimensional (2D) and three-dimensional (3D) structures of chicken major histocompatibility complex (MHC) class I protein (
BF2
and beta2m) and further reconstruct their complex identifying the virus-derived antigenic peptides, the mature protein of
BF2
and beta2m genes were expressed solubility in pMAL-p2X/Escherichia coli. TB1 system. The expressed MBP-
BF2
- and MBP-beta2m-fusion proteins were purified, and cleaved by the
factor Xa
protease. Subsequently, the monomers were further separated, and the purified MBP-
BF2
, -beta2m, and MBP were analyzed by circular dichroism (CD) spectrum. The contents of alpha-helix, beta-sheet, turn, and random coil in
BF2
protein were 72, 102, 70, and 90 amino acids (aa), respectively. The beta2m proteins displayed a typical beta-sheet and the contents of alpha-helix, beta-sheet, turn, and random coil were 0, 46, 30, and 22 aa, respectively. Homology modeling of
BF2
and beta2m proteins were similar as the 3D structure of human MHC class I (HLA-A2). The results showed that pMAL-p2X expression and purification system could be used to obtain the right conformational
BF2
and beta2m proteins, and the 2D and 3D structures of
BF2
and beta2m were revealed to be similar to human's. The recombinant
BF2
and beta2m-based proteins might be a powerful tool for further detecting antigenic peptides.
...
PMID:Structures and homology modeling of chicken major histocompatibility complex protein class I (BF2 and beta2m). 1611 97
