Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.6 (
thromboplastin
)
13,278
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Human monocytes cell extracts have been analysed by density gradient centrifugation in Percoll gradients. Two peaks of activity of the plasma membrane marker enzyme
5' nucleotidase
were detected. The main peak was at a density of 1.040 and a secondary one at a lower density. Thromboplastin activity was recovered associated with the main peak of
5' nucleotidase
activity in control cells. In NH4Cl treated cells,
thromboplastin
activity is found at both densities. In agreement with this, labelling of the cell surface with 125I shows a reduction in availability of proteins for labelling between control and NH4Cl treatment. The availability of
thromboplastin
in monocytes for attack by exogenous trypsin or for initiation of coagulation is also reduced by cultivation with amines. We suggest that the low density peak of 5'nucleotidase and
thromboplastin
represents an intracellular compartment.
...
PMID:An intracellular pool of the procoagulant thromboplastin in human monocytes. 300 17
