Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.6 (
thromboplastin
)
13,278
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Factor Xa has been reported to elicit smooth muscle cell proliferation via autocrine release of platelet-derived growth factor. However, this study has shown that
factor Xa
-induced mitogenesis of rat aortic smooth muscle cell is independent of platelet-derived growth factor. We also could not observe any platelet-derived growth factor isoforms in the cultured medium of
factor Xa
-stimulated cells. Our finding that the cultured medium of
factor Xa
-stimulated cells strongly induces rat aortic smooth muscle cell mitogenesis in the absence of
factor Xa
activity led us to explore the existence of a novel autocrine pathway. The autocrine growth factor was purified from the cultured medium and was identified to be
epiregulin
. Recombinant
epiregulin
was also able to induce the mitogenesis. The secretion of
epiregulin
from
factor Xa
-stimulated rat aortic smooth muscle cell required mRNA expression and protein synthesis of the growth factor. The mitogenic effect of
factor Xa
on rat aortic smooth muscle cell was significantly reduced by anti-
epiregulin
antibody or by antisense oligodeoxynucleotide to
epiregulin
. Several lines of experimental evidence clearly indicate that the autocrine production of
epiregulin
, an epidermal growth factor-related ligand, is induced in the
factor Xa
-stimulated mitogenic process of rat aortic smooth muscle cell.
...
PMID:Factor Xa induces mitogenesis of vascular smooth muscle cells via autocrine production of epiregulin. 1457 Aug 97
