Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.6 (thromboplastin)
 13,278 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The sensitivity of the standard prothrombin time (PT) was low when measured on 56 plasmas with a reduced activity of the coagulation factors mainly of the prothrombin complex (factors II, VII and/or X) taken from eight dogs at different times of vitamin K1 therapy after coumarin intoxication. This was demonstrable by use of three different Ca-thromboplastins. With the standard test only plasmas with an excessive decrease of coagulation factor activity (sum of activity decrease of the single factors in relation to the respective reference range [SAD] > 100%) were detectable with sufficient reliability. In contrast, by using a method optimized for dogs (1:20 sample predilution, fibrinogen substitution) and respecting the species specific features of coagulation physiology, pathological PT-values were measured in up to 70% of the samples (dependent on the Ca-thromboplastin) with slight reductions of single factor activity (SAD: 11-25%). Significant differences concerning the sensitivity were seen additionally between the different Ca-thromboplastins. Human placenta thromboplastin in particular, but also rabbit brain thromboplastin, were more sensitive than a preparation of recombinant human tissue factor. The correlation between the PT and the SAD was closer when using the optimized method (r = 0.919-0.954) compared to the standard test (r = 0.771-0.862). In contrast to the standard test, the PT optimized for dogs is, therefore, a reliable screening test to recognize a slight reduction in prothrombin complex. It is especially suitable for monitoring of vitamin K1 therapy after coumarin intoxication.
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PMID:Evaluation of a prothrombin time optimized for the dog on plasmas with defined coagulation factor deficiency due to coumarin intoxication. 882 95