Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.21.5 (thrombin)
 33,306 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of ultraviolet radiation (UV-A, 360 nm) on the thrombin-induced aggregation of washed pig platelets as well as on the release of adenine nucleotides and proteins was studied. The level in platelets of adenine nucleotides, mainly ADP and ATP, decreased rapidly following the exposure of platelets to a high dose of UV-A (0.5 W/cm2, 30 min). Through thrombin-induced aggregation of irradiated platelets was inhibited, the release reaction occurred. The amount of the released adenine nucleotides and proteins was, however, dependent on the dose of UV light. These findings suggest that UV-A light can stimulate the platelet release reaction.
Acta Biochim Pol 1985
PMID:UV light-induced changes in washed pig platelets. 403 49

Craviten effect on platelets suspended in their own plasma was investigated by two methods of incubation with the drug: the fresh platelets were incubated for 18 h at 4 degrees C with Craviten added to plasma and platelets stored for 18 h at 4 degrees C without Craviten were incubated with Craviten at 37 degrees C for 1 h. For evaluation of the metabolic activity and function of the platelets, the levels of high-energy compounds ATP and ADP, the activity of hexokinase and pyruvate kinase and the amount of adenyl nucleotides released by the platelets after thrombin addition were measured, and the spontaneous aggregation of platelets and c-AMP were determined. The experiments demonstrated that Craviten prevented the fall of ATP level of the stored platelets, raised the activity of hexokinase and pyruvate kinase in the platelets, increased the amount of nucleotides released by the platelets in the release reaction. Craviten inhibited also increased spontaneous aggregation of stored platelets and raised c-AMP level.
Pol J Pharmacol Pharm
PMID:Craviten effect on the metabolism of stored platelets. 718 49

Aspirin (ASA) is widely used in the treatment of cardiovascular diseases. Recently, we have found that aspirin decreases not only platelet aggregation but also thrombin generation. This effect, however, was seen only in certain subjects. Therefore we decided to examine influence of a single dose of aspirin (500 mg) on thrombin generation in healthy volunteers. Thrombin genesis was assessed by serial measurements of fibrinopeptide A concentration in blood emerging from standardised forearm skin incisions. Aspirin reduced thrombin generation in persons with normal serum level of lipids. This effect was lost, however, in subjects with high level of cholesterol and lipoprotein (a)--well known risk factors of ischaemic heart disease. While the mechanism by which aspirin affects thrombin generation remains to be elucidated, our data indicate that hypercholesterolemic subjects might benefit less than others from preventive aspirin treatment.
Pol Arch Med Wewn 1995 Jun
PMID:[High levels of cholesterol and lipoprotein (A) in serum decreases the inhibitory effect of aspirin on generation of thrombin]. 749 48

The thrombin clotting time and the fibrin polymerization test were performed in 64 patients with liver cirrhosis. Each test was made with thrombin diluted with 150 mmol/l sodium chloride and 25 mmol/l calcium chloride. The thrombin-in-NaCl clotting time was found to be prolonged in 39% of cirrhotic patients, whereas fibrin polymerization was defective in 65% of them. Dilution of thrombin with calcium chloride diminished the number of abnormal results of both tests in patients with liver cirrhosis. Among the performed tests most sensitive test proved to be the polymerization of fibrin induced by thrombin diluted with sodium chloride.
Mater Med Pol
PMID:Thrombin clotting time and fibrin polymerization in liver cirrhosis. 760 83

The activity of serum alpha-6-fucosyltransferase, a platelet derived enzyme, determined in sera of 22 normal individuals and 86 patients with various disorders was positively correlated with platelet counts. When the enzyme activity in 1 microliters serum was calculated per 1000 of platelets in blood (coefficient F/P) an inverse correlation became evident in that F/P was proportionally the higher the lower was platelet count in blood. The F/P values were in a good agreement with the results of direct assays of enzyme activities in isolated platelets. Neither granulocytes, lymphocytes nor red cells significantly contributed to serum enzyme activity though granulocytes enhanced the thrombin-induced enzyme release from platelets. In platelets separated by centrifugation in density gradients the enzyme was shown to be present in platelets of intermediate and high density but missing from the light ones. It is suggested that alpha-6-fucosyltransferase of platelets may be a marker of the ploidy level of megakaryocytes.
Acta Biochim Pol 1995
PMID:Activity of platelet alpha-6-fucosyltransferase is inversely related to blood platelet concentration. 765 58

In 25 patients with atherosclerosis of lower limb arteries (ALLA) in whom vascular operation was performed, the level of thrombin-antithrombin III (TAT) complexes and antithrombin III (AT III) activity were determined in comprehensive studies of the blood clotting system. The comparative group for the assessment of operation effect on primarily not disturbed haemostasis in humans included 10 men operated on for inguinal hernia, while the control group consisted of 54 healthy people. Blood for the determinations was taken before the operation, on the day of the operation (0-30 minutes after the operation), on the first day after it, and also on the third day in patients with hernia, and on the 7th day in patients with ALLA. In the patients with ALLA, blood hypercoagulation features were found, expressed as increased level of TAT complexes in spite of lack of abnormalities in comprehensive coagulation studies, while in the patients with hernia only increased AT III activity was found. The surgical operation caused in the patients with ALLA enhancing of hypercoagulation which was evidenced by over threefold increase of TAT complexes on the day of the operation.
Pol Tyg Lek
PMID:[Effect of vascular surgery on values of thrombin-antithrombin III complexes in patients with atherosclerosis of lower limb arteries]. 770 52

Five new dipeptides containing epsilon-aminocaproic acid were synthesized. All dipeptides markedly inhibit fibrinolytic activity of plasmin but only in low concentration (0.0002M). In higher concentration fibrinolytic activity was observed. Activity of dipeptides containing epsilon-aminocaproic acid, which antifibrinolytic activity is known, was tested on plasmin, thrombin, urokinase and euglobulin fraction using synthetic substrates.
Acta Pol Pharm 1994
PMID:Synthesis and activity of dipeptides containing epsilon-aminocaproic acid. 776

It was found that storing the plasma in the temperature 0 degree C, 4 degrees C and 20 degrees C and especially in 37 degrees C increases pH and decreases antiheparin activity estimated by the heparin-thrombin test. Measurements of the heparin-thrombin time should be carried out directly after obtaining the plasma or after reducing the plasma to pH 7.40.
Ann Med Univ Bialyst Pol 1993
PMID:Complexoproductive and antiheparin properties of low density lipoproteins (LDL). V. Optimum conditions of the coagulation method to determine antiheparin activity in the blood plasma. 792 85

The relationship between thrombosis and atherosclerosis has been already suggested in the middle of the last century. More recently, in 1976 a hypothesis has been put forward which emphasize the leading role of chronic injury to endothelial cells followed by platelet attachment and the release of platelet derived growth factors in pathogenesis of atherosclerosis. There is also a growing body of evidence that cytokines, thrombin and the fibrinolytic system are involved in the initiation and progression of atherosclerotic lesions. Lipoprotein (a) is emerging as a link between atherogenic role of lipids and the haemostatic system. At the same time epidemiological data are pointing at the possible role of fibrinogen, factor VII and plasminogen activator inhibitor-1 as risk factors for ischemic heart disease. Thrombus formation is also responsible for the majority of acute coronary syndromes. On the other hand aspirin and other antiplatelet drugs seem to protect from vascular complications of atherosclerosis. Than the question which rather arises is: what is the best antithrombotic strategy in patients with cardiovascular diseases?
Pol Tyg Lek
PMID:[Should hemostatic factors be considered in the prevention of cardiovascular disease?]. 797 94

Plasminogen activator inhibitor-1 (PAI-1) plays particularly important role in regulation of homeostasis of fibrinolytic system. It neutralizes active molecules of tissue-type and urokinase-type plasminogen activators. PAI-1 is synthesized mainly in endothelial cells but it is present also in other cells. It was found in vitro that elevated expression of PAI-1 gene and increase in PAI-1 concentration released from endothelium is caused by many different biologically active substances. Among them there are thrombin, lipopolysaccharides, cell growth factors, cytokines and also glucocorticoids and phorbol esters. In this work mechanisms of regulation of PAI-1 synthesis and role of this protein in homeostasis of fibrinolytic system are described.
Acta Haematol Pol 1994
PMID:[Progress in knowledge about fibrinolysis regulation]. 799 70


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