Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.5 (thrombin)
 33,306 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The role of phospholipase C (an enzyme involved in the metabolism of inositol-containing phospholipids), cyclooxygenase and lipoxygenase (the enzymes of arachidonic acid metabolism), and adenylate cyclase and phosphodiesterase (the enzymes of cyclic adenosine 3,5-monophosphate (cAMP) metabolism) in the mechanisms of the aggregation of human platelets induced by the serine protease in low concentrations (thrombin 0.5 mkg per ml, trypsin 1 mkg per ml, and alpha-chymotrypsin 10 mkg per ml) have been investigated with the use of the inhibitor analysis. The effect of neomycin sulfate (phospholipase C inhibitor), indometacin (cyclooxygenase inhibitor), and nordihydrogvayaretic acid (lipoxygenase inhibitor) on protease-induced increase in the content of calcium cations in platelet plasma has been studied. The results of the inhibitor analysis indicated that the enzymes of metabolism of inositol-containing phospholipids, arachidonic acid, and cAMP are involved in the mechanisms of the protease-induced platelet aggregation. The increase in the content of calcium ions, associated with the protease-induced activation of phospholipase C, in cytoplasm may play an important role in the mechanisms of platelet aggregation.
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PMID:[Mechanisms of the protease-induced aggregation of human platelets]. 1790 8

The bioactive protein components from snake venom complexes have been utilized for studies of enzymology, structural biology, and pharmacology. The Gloydius shedaoensis snake (GSS) is the only snake species found exclusively at the Chinese Shedao (snake) Island in Dalian. To investigate the protein components of Chinese GSS venom (GSSV), we initialized a proteomic assay for GSSV by the combination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis with high-performance liquid chromatography (HPLC)-nanoelectrospray ionization tandem mass spectrometry (nESI-MS/MS). Thirty gel bands visualized by Coomassie blue staining were excised and digested by trypsin. The tryptic-digested peptides were separated by HPLC and subsequently sequenced by nESI-MS/MS. Twenty-four types of proteins were identified by searching the mass spectrometry data against NCBInr database through TurboSequest Bioworks. The most abundant proteins are phospholipase A(2) , metalloproteinase, L-amino acid oxidase (LAAO), serine protease/thrombin-like enzyme. Except for 20 types of known snake venom proteins, the homolog peptides of hypothetical protein PFLC2230, LOC495267 protein, DEAD/DEAH box helicase-like, and pancreatic trypsin 1 from other organisms are matched for GSSV protein components. Mass spectrometric data also indicated that (i) dimerization happens to PLA(2) s as monomer and dimer of PLA(2) s coexist in GSSV and (ii) truncation or hydrolysis might happen to LAAOs as three molecular-weight-ranged LAAO species are present in GSSV. The results provide an "anatomical" view of the protein composition and important information for protein characteristics of GSSV.
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PMID:"Anatomical" view of the protein composition and protein characteristics for Gloydius shedaoensis snake venom via proteomics approach. 2123 2