Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.4 (trypsin)
 42,187 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Psoriatic plaque scale was collected from untreated patients, homogenized, and subjected to chromatography on Sephadex and DEAE-cellulose to separate proteolytic enzymes. Five proteolytic enzymes were partially separated and characterized as to their substrate specificities, pH-optimum and inhibitor characteristics. A neutral trypsin-like protease, an alkaline histone hydrolyzing protease and a neutral chymotrypsin-like protease isolated were found to differ from those separated earlier from normal human skin using the same procedures for enzyme purification. The trypsin-like enzyme preparation was found to be an effective fibrinolytic activator and the histone hydrolyzing enzyme showed fibrinolytic activity. The possible roles of these proteases in the increased cell division rate of psoriatic plaque are discussed.
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PMID:Plasminogen activator and histone hydrolyzing proteases in psoriasis scales--possible role in increased cell division. 99 19

Psoriatic plaque contains an increased number of mast cells that are thought to play an important role in the initiation and maintenance of the disease through the release of mediators such as histamine, proteoglycans, proteinases and cytokines. To verify the possible participation of these cells in the chronic inflammatory cutaneous response in psoriasis, we performed a double-blind controlled study to investigate the presence and activation of tryptase-positive mast cells in the lesional skin of 19 patients affected by active psoriasis vulgaris minima compared with five healthy, age-matched subjects. Psoriatic patients were randomized into two groups (A and B). The first group was treated with cetirizine (10 mg/three times a day for 15 days) and the second one was treated with placebo. Both groups underwent clinical staging [psoriasis area and severity index (PASI) score] and immunohistochemical evaluation [alkaline phosphatase antialkaline phosphatase (APAAP) procedure] before and after treatment. In group A, the PASI score ranged from 3.8 (SE +/- 1.00) to 1.8 (SE +/- 0.68) and in group B, from 5.0 (SE +/- 0.98) to 3.4 (SE +/- 0.47). The mean number of tryptase-positive mast cells for field, mainly distributed in the perivascular and periadnexal sites, ranged from 40.8 (SE +/- 7.15) to 21.6 (SE +/- 3.04) in group A and from 25.1 (SE +/- 3.78) to 26.3 (SE +/- 3.59) in group B (ANOVA test f = 6.95; gl = 1.16; p = 0.02). In our psoriatic patients, cetirizine significantly reduced the expression of tryptase-positive mast cells and produced a clinical improvement in erythema, suggesting a multilevel immunopharmacologic modulation of this antihistamine in psoriasis.
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PMID:Cetirizine reduces the number of tryptase-positive mast cells in psoriatic patients: a double-blind controlled study. 1151 56