Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.4 (trypsin)
 42,187 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We examined the cellular distribution of rat tryptase in rat skin, lung, small intestine, and peritoneal lavage cells by immunohistochemical techniques. Tryptase purified to apparent homogeneity from rat skin was used to generate a goat polyclonal anti-rat tryptase antibody. Tryptase-containing cells were detected in lung, skin, and peritoneal lavage cells. Small intestine mucosa, on the other hand, showed few if any tryptase-positive cells. Sequential staining with Alcian blue and anti-tryptase antibody showed that tryptase is located only in mast cells. Sequential staining with safranin to identify the connective tissue type of mast cell and anti-tryptase antibody showed that tryptase resides only in this mast cell type. However, only a subpopulation of the safranin-stained mast cells contained tryptase. In lung, 53% of the mast cells stained with safranin; 94% contained tryptase. In skin, 80% stained with safranin; only 6% contained tryptase. In peritoneal cells, more than 95% of the mast cells were stained with safranin; 20% contained tryptase. In the bowel mucosa, where few cells are stained by safranin, no cells with tryptase were detected. The percentages of cells with chymase I that also contained tryptase were 80% and 84% for lung, 4% and 7% for skin, and 15% and 13% for peritoneal cells by respective simultaneous and sequential double labeling with anti-tryptase and anti-chymase I antibodies. This study suggests that the rat connective tissue type of mast cell is subdivided into two forms on the basis of the presence or absence of tryptase, whereas rat mucosal mast cells lack this enzyme. These results contrast with those in humans, in which tryptase is present in all mast cells, but are similar to mice, in which tryptase mRNA has been detected only in the connective tissue type.
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PMID:Localization of rat tryptase to a subset of the connective tissue type of mast cell. 768 89

Digestion, enzyme secretion and intestinal rate of passage were determined in broiler chicks from hatch until 21 d using 141Ce as a nonabsorbed reference substance. Body weight and feed intake increased more rapidly after 10 d posthatch, and, in parallel, time of passage of feed through the intestines decreased by approximately 33%. Net duodenal secretion of amylase, trypsin, and lipase was low at 4 d and increased 100-, 50-, and 20-fold, respectively, by 21 d. Enzyme activity decreased distally in the small intestine. This change was greater with age. The contribution of the ileum to fatty acid absorption decreased after 7 d. Small intestine digestion of nitrogen increased from 78% at 4 d to 92% at 21 d, whereas fatty acid and starch digestion ranged from 82 to 89% in this period. It appears that digestibilities of starch and lipids are not limiting factors in the growth of young chicks.
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PMID:Digestion and absorption in the young chick. 772 61