Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.21.4 (trypsin)
 42,187 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three other isolates were strongly cytotoxic for 3T3 cells but only slightly so for BRL 3A cells. The remaining three isolates showed no cytotoxicity for either cell line. The cytotoxic products were greater than 100 kDa in mol. wt, thermolabile, and partly destroyed by trypsin treatment. The data show that some B. piliformis isolates produce cytotoxic proteins, which may contribute to the pathogenesis of Tyzzer's disease.
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PMID:Cytotoxicity of Bacillus piliformis. 162 20

Spores of Bacillus piliformis of rabbit origin were harvested from the yolk sac of previously inoculated hen's eggs and subjected to various heat or chemical disinfectant treatments. Subsequently, spores were tested for infectivity in embryonated eggs inoculated via the yolk sac route, and others were treated with trypsin and then tested in embryonated eggs. The spores were not affected by the heat treatment of 60 degrees C but were rendered noninfective with treatments of 70 degrees C and 80 degrees C. Infectivity was not restored by treatment with trypsin. Infectivity was not lost when spores were treated with a phenolic germicidal detergent, ethanol or either of two quaternary ammonium compounds containing 9% or 17% benzalkonium chloride. A graded effect was observed with formaldehyde solution and an iodophor. Spores were rendered noninfective after treatment with peracetic acid (1.0%) and a wetting agent, sodium alkylarylsulfonate or sodium hypochlorite solution (0.3%) for 5 minutes, and infectivity was not restored by trypsin treatment. The probable means of transmission of Bacillus piliformis was discussed and sodium hypochlorite solution (0.3%) was recommended as a surface disinfectant in animal facilities as an aid to the prevention and control of Tyzzer's disease.
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PMID:Effect of heat and selected chemical disinfectants upon infectivity of spores of Bacillus piliformis (Tyzzer's disease). 705 74