Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.4 (trypsin)
 42,187 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Acetylcholine receptors (AChRs) accumulate at developing neuromuscular junctions in part via lateral migration of diffusely expressed receptors. Using a model system--cultured Xenopus muscle cells exposed to electric fields--we have shown that AChRs, concentrated at the cathode-facing cell pole, continue to aggregate there after the field is terminated (Stollberg and Fraser, 1988). These observations are consistent with the possibility that the field-induced increase in receptor concentration triggers the aggregation event. Only 2 other molecular events could initiate the electric field-induced receptor aggregation: (1) a local increase in the density of some other molecules, or (2) a voltage-sensitive mechanism. Treatment of muscle cell cultures with neuraminidase changes the cell surface charge and has been reported to reverse the direction of electromigration for AChRs and concanavalin A binding sites (Orida and Poo, 1978). Using digitally analyzed fluorescence videomicroscopy, we find that AChRs in neuraminidase-treated cultures accumulate at both cell poles in an electric field. After termination of the field, the AChR continues to aggregate at the cathode-facing pole, as in cells not treated with neuraminidase. However, receptor density decreases at the anode-facing pole, indicating that elevated AChR density does not initiate receptor aggregation. Cells pretreated with neuraminidase and trypsin (which blocks receptor aggregation) display reversed receptor distributions compared to untreated controls, indicating that electromigration has indeed been reversed. The rate at which neuraminidase- and trypsin-treated cells approach steady-state distributions indicates a receptor diffusion constant of approximately 1.2 x 10(-9) cm2/sec, consistent with a diffusion trap mechanism of receptor aggregation.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Local accumulation of acetylcholine receptors is neither necessary nor sufficient to induce cluster formation. 229 95

Fraser, C. E. Ovid (University of Wisconsin, Madison), and David T. Berman. Type-specific antigens in the psittacosis-lymphogranuloma venereum group of organisms. J. Bacteriol. 89:943-948. 1965.-Antigens of 14 strains of the psittacosis-lymphogranuloma venereum (PLV) group of organisms were prepared by treating purified particles with deoxycholate and trypsin. In complement-fixation tests of these antigens with the homologous and heterologous antisera, specific serotype differences were observed. Application of the method of specificity differences permitted placement of the 14 strains into 7 subgroups. The possible value of these techniques in immunology, epizootiology, and taxonomy of the PLV group is discussed.
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PMID:TYPE-SPECIFIC ANTIGENS IN THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM GROUP OF ORGANISMS. 1427 19