Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.4 (trypsin)
 42,187 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Rabbits were injected intraarticularly three times with 0.5 ml of 10, 50 or 100 mg% purified rheumatoid synovial collagenase or with identical amounts of trypsin. 18 hours after the last injection the collagenase-injected animals showed distinct cellular exudation into the synovial fluid and acute arthritis; one week later there was a decrease of the cell count in the synovial fluid and appearance of proliferative synovitis, while 3 weeks later there was no exudation and less chronic inflammation but distinct fibrosis of the synovium. A direct action of collagenase on the connective tissue components would seem to be responsible for these changes. No pathologic alteration of the cartilage was observed. Trypsin-injected control animals showed negligible cellular exudation and no pathologic alteration of the synovium.
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PMID:Collagenase-induced experimental arthritis. 21 3

Purified human granulocyte collagenase (1 mg %, 10 mg % or 50 mg %) was injected into rabbit knee joints (three groups of 6 animals each) three times within one week. Synovium and synovial fluid were investigated 18 hours, 1 week and 3 weeks after the last injection. After 18 hours, synovial fluids showed distinct cellular exudation, its size depending on the amount of collagenase applied. A smaller number of cells was seen after one week, while normal cell counts were observed 3 weeks after the last injection. Histologically, synovium showed an acute arthritis after 18 hours, whereas after 1 and 3 weeks a chronic proliferative form of arthritis with predominant activation of fibroblasts was diagnosed. As compared with an experimental arthritis induced with rheumatoid synovial collagenase, granulocyte collagenase was less arthritogenic. Neither trypsin nor saline injections induced distinct cellular exudation into synovial fluids nor histologic signs of arthritis.
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PMID:Experimental arthritis induced by granulocyte collagenase. 625 49