Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.1 (
chymotrypsin
)
10,938
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
HBp17
was purified by Heparin-Copper biaffinity chromatography and HPLC from conditioned medium of A431 cell. The purified
HBp17
was digested by staphylococcus urcus V8 protease or
chymotrypsin
and the heparin-binding fragments were isolated by Heparin-Sepharose. One binding site of peptide mapping is
HBp17
residues 110-145 produced by V8. Another one is
HBp17
residues 82-143 which were produced by
chymotrypsin
digestion. Two binding sites of peptide mapping are overlap. Therefore the residues 110-143 of
HBp17
are the principle heparin binding site. The basic amino acid cluster in this region may be contribute to the binding of
HBp17
to heparin or heparan sulfate proteoglycan on the cell surface and extracellular matrix.
...
PMID:Purification of heparin-binding protein HBp17 and identification of HBp17 heparin binding site. 978 42
