Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.21.1 (
chymotrypsin
)
10,938
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The reaction of tris(2-chloroethyl)amine (
TCEA
) with purified hemoglobin and its effect on properties of hemoglobin was studied using 14C-labeled
TCEA
. Hemoglobin remained soluble after binding as much as 4
TCEA
per heme. In concentrations which did not denature hemoglobin
TCEA
reacted only with a small proportion of the free SH groups; blockade of the SH groups with PMB did not noticeably affect the binding of
TCEA
to hemoglobin. Hydrolysis by trypsin or
chymotrypsin
of hemoglobin which had reacted with
TCEA
yielded radioactive peptides besides not radioactive peptides and radioactive compounds not reacting with ninhydrin. The reaction with
TCEA
caused a change in electrophoretic mobility of hemoglobin and prevented its complete disintegration by PMB into subunits. After reaction with
TCEA
the affinity of hemoglobin for oxygen was strongly increased and the heme-heme interaction strongly diminished. The Bohr effect and the effect of 2,3-diphosphoglycerate on oxygen affinity remained unchanged. The effect of
TCEA
on the properties of hemoglobin points to specificity in its reaction with functional groups of hemoglobin.
...
PMID:The effect of tris(2-chloroethyl)amine on human hemoglobin. 1 12
