Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.21.1 (chymotrypsin)
 10,938 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Adult rabbit zonular fibers maintained in their native condition were treated with collagenase, alpha-chymotrypsin and hyaluronidase, and were observed with the electron microscope. The results obtained were as follows: 1. Collagenase digested the lens capsule, but not the zonular fibers. 2. Long time collagenase action obscured the cell membrane of the lens epithelium and the basal lamina of the ciliary epithelium. 3. Washing with 0.9% NaCl increased the collagenase action on the lens capsule. 4. Alpha-chymotrypsin digested the zonular fibers and the zonular lemalla, but not the lens capsule and the basal lamina of the ciliary epithelium. 5, Hyaluronidase only slightly changed the lens capsule. 6. The vitreous fibers were digested by collagenase, but not by alpha-chymotrypsin or hyaluronidase. Thes results together with the review of recent literature indicate that the zonular fiber has a nature close to that of the microfibril of elastic fiber.
 ...
PMID:Electron microscopic studies on zonular fibers. II. Changes of the zonular fibers after the treatment with collagenase, alpha-chymotrypsin and hyaluronidase. 16 41

Studies have been made on the effect of trypsin, chymotrypsin, pronase, lipases, hyaluronidase and digitonin on electrophysiological properties of the neurons of the snail H. pomatia under external application. Proteases and lipases gradually depolarize the neuronal membrane, decrease the amplitude and prevent the onset of action potentials, initially increase and then decrease the membrane resistance. The decrease in the membrane resistance coincides with the period of maximum inhibition of resting and action potentials in the neurons. The enzymes studied do not affect the membrane capacitance. Changes in electrophysiological characteristics induced by the enzymes are partially reversible provided the preparation is soaked in Ringer's solution for a sufficient time. Digitonin rapidly and irreversibly depolarizes the membrane, decreases its resistance and blocks action potentials. Hyaluronidase does not significantly affect neuronal electrophysiological properties when applied solely, but facilitates the development of changes during subsequent effect of proteases.
 ...
PMID:[Effect of hydrolases and digitonin on the electrophysiological characteristics of the neurons of the snail, Helix pomatia]. 67 79

Isolation of cells is nowadays performed by enzymatic means. The influence of such enzymes on the surface coat of mesenchymal and blastemal cells during the dissociation of limbs buds from 11-day-old mouse embryos was studied electron microscopically after staining with ruthenium red. EGTA or collagenase failed to bring about cell separation. The surface coat seemed to be unchanged after collagenase treatment. After EGTA an increase in extracellular filaments was observed. The proteases alpha-chymotrypsin, dispase II, papain, pronase P and trypsin (0.2%, 37 degrees C, 20 min) succeeded in completely dissociating limb buds. Apart from single granules, there was a detachment of the surface coat from the cells in all cases studied. Hyaluronidase led to only partial separation, but the detachment of the surface coat was almost complete, indicating a GAG-rich surface layer on these cells.
 ...
PMID:Changes in the surface coat of mesenchymal cells of mouse limb buds after enzymatic cell separation. 626 Aug 88