Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.17.17 (tubulin carboxypeptidase)
 42 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cat brain carboxypeptidase releasing C-terminal arginine from the synthetic substrate, dansyl-Phe-Leu-Arg, was partially characterized. The enzyme has a molecular weight of 100-120 kDa, displays the maximal activity at pH 6.0-6.5 and is strongly inhibited by phenylmethanesulfonylfluoride and 4-chloromercuribenzoate, less strongly (by 40%) by iodoacetamide and is not inhibited by N-ethylmaleimide, 2-mercaptoethanol, EDTA, Co2+ and guanidinoethylmercaptosuccinic acid. The Km values for the hydrolysis of dansyl-Phe-Leu-Arg and dansyl-Phe-Ala-Arg by soluble carboxypeptidase are 48 and 96 microM, respectively. By all properties, this carboxypeptidase differs from other known carboxypeptidases. Possible participation of the enzyme in neuropeptide metabolism is discussed.
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PMID:[Partial characteristics of the basic phenylmethylsulfonylfluoride-inhibited carboxypeptidase from cat brain]. 859 Jul 58