Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.16.2 (
PCP
)
3,761
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The fluorophore 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl carbocyanine iodide (JC-1) localizes to the mitochondria and is affected by membrane potential, fluorescing bright orange when the membrane potential is high and green when mitochondrial membrane potential is low. The present study used flow cytometric analysis of JC-1 staining patterns of large numbers of
spermatozoa
to detect chemical-induced alterations of sperm mitochondrial membrane potential. Cauda epididymal rat
spermatozoa
were incubated with pentachlorophenol (
PCP
; 0.1 microM or 1.0 microM), a known uncoupler of mitochondrial oxidative phosphorylation. Microscopic evaluation showed that the midpiece (mitochondrial location) of live, highly motile
spermatozoa
stained bright orange, while the midpiece of live, non-motile
spermatozoa
stained green. The midpiece of slightly or non-progressively motile
spermatozoa
stained a faint orange-green. The percentage of
spermatozoa
stained bright orange and the total percentage of
spermatozoa
stained orange (bright orange+faint orange) in the control samples of
spermatozoa
were significantly higher (P<0.001) than in the 0.1 microM and 1.0 microM
PCP
treated samples. These data indicate that sperm mitochondrial membrane potential is highly sensitive to the uncoupling effects of
PCP
and that JC-1 staining and flow cytometric analysis may be a sensitive assay to detect the effect of toxicants on rat sperm mitochondrial function.
...
PMID:Flow cytometric assessment of changes in rat sperm mitochondrial function after treatment with pentachlorophenol. 1278 Dec 3
